Calcium silicate particles containing mesoporous SiO2 (CaSiO3@SiO2) on the surface were grafted onto polypropylene nonwoven. The PP nonwoven grafted CaSiO3 containing mesoporous SiO2 (PP-g-CaSiO3@SiO2) was characterized by TEM and TG. The adsorption behaviors of bisphenol A (BPA) on PP-g-CaSiO3@SiO2 were investigated. The results indicated that the large surface area and the Si–OH groups of the material improved the adsorption capacities and affinity for BPA. The adsorption rate was fast, and the adsorption capacity was high, even with the background organic compound alginate sodium. The adsorption mechanism of BPA by PP-g-CaSiO3@SiO2 was investigated. Isothermal titration calorimetry (ITC) was used to illustrate the driving force for the adsorption of BPA. PP-g-CaSiO3@SiO2 was prepared simply without using any organic solvent and could be recycled and reused.

•BSA imprinted CA/PAM hydrogel film with high toughness was prepared.•The regeneration property of BSA imprinted CA/PAM hydrogel was significantly improved.•Excellent recognition performance of the CA/PAM MIP was proved by single and binary proteins rebinding experiment.•CA/PAM MIP is low-cost, easy to be prepared and has potential application in cell culture.•The eluent SDS should be avoided in preparing MIP for cell culture.Protein imprinted calcium alginate/polyacrylamide hydrogel film (CA/PAM MIP) with high toughness was prepared using bovine serum albumin (BSA) as template molecule, sodium alginate and acrylamide as functional monomers, N,N′-methylenebisacrylamide (MBAA) as the covalent cross-linker and CaCl2 as the ionic cross-linker via UV radiation-reduced polymerization. Factors affecting the adsorption capacity and imprinting efficiency of the BSA-imprinted CA/PAM hydrogel films were investigated, such as ratio of polyacrylamide/sodium alginate, film thickness, MBAA concentration and CaCl2 concentration. Results showed that the CA/PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA compared with non-imprinted polymer (NIP). The adsorption capacity of MIP for BSA reached 22.49 mg/g, which was 2.7 times higher than NIP. The regeneration property of the BSA-imprinted CA/PAM hydrogel was distinctly improved and the imprinting efficiency of CA/PAM MIP maintained 77.95% of the initial value after five repetitions. Single and binary proteins rebinding indicated that the CA/PAM MIP exhibited good recognition performance. Cell culture experiments showed CA/PAM MIP was more suitable for cell culture than CA/PAM NIP. The residual sodium dodecyl sulfate (SDS) in the elution process leaded to the death of mouse fibroblast cells (L929) after 3 days. A moderate elution solution without residue eluent should be used to prepare MIP for cell culture.

•BSA imprinted PP fiber-grafted PAM hydrogel membrane (PP-g-PAM MIP) was prepared.•PP fiber grafted PAM hydrogel was characterized by SEM, FT-IR, DSC, TG and DTG.•Influence factors on the adsorption capacity of PP-g-PAM MIP and NIP were investigated.•The regeneration property of PP-g-PAM MIP was improved.•The PP-g-PAM MIP could extract required proteins from cells or particulate samples.Bovine serum albumin (BSA) imprinted polypropylene (PP) fiber-grafted polyacrylamide (PAM) hydrogel membrane (PP-g-PAM MIP) was prepared using non-woven PP fiber as matrix, BSA as template molecule, and acrylamide (AM) as functional monomer via UV radiation-reduced polymerization in an aqueous phase. SEM, FT-IR, DSC and TG were used to characterize the PP grafted PAM hydrogel. Influence factors on the adsorption capacity of PP-g-PAM MIP were investigated, such as monomer concentration, cross-linker concentration, template molecule amount and pH values in BSA solution. The adsorption and recognition properties of PP-g-PAM MIP were evaluated and the results showed that the PP-g-PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA as compared with non-imprinted ones. PP-g-PAM MIPs could recognize the template protein using Lys, Ova, BHb, and Glo as control proteins, and the selectivity factor (β) was above 2.0. The imprinting efficiency of PP-g-PAM MIP tended to be stable after three cycles and maintained 76% of the initial value of the imprinting efficiency even after five repetitions, which was more excellent than that of PAM microsphere. The PP-g-PAM MIP is low cost and easy to be prepared, which would show its potential applications in the fields of extracting and testing required proteins from cells or particulate samples.The figure shows that the imprinting efficiency of PP-g-PAM MIPs gradually decreased with the cycle times (from 3.30 to 2.56) and reached a stable value after three cycle times. The imprinting efficiency of PP-g-PAM MIPs was maintained at 76% of the initial value even after five cycles. However, the imprinting efficiency of BSA-imprinted PAM microspheres continually declined with increasing cycle times. After five cycle times, the imprinting effect almost disappeared. This result is probably due to the soft texture of gel microspheres, which cannot stabilize the shape of the binding sites. By contrast, the strength of PP-g-PAM MIPs was significantly improved and the shape of the binding sites was almost stabilized.

•BSA imprinted CA/PAM hydrogel film with high toughness was prepared.•The regeneration property of BSA imprinted CA/PAM hydrogel was significantly improved.•Excellent recognition performance of the CA/PAM MIP was proved by single and binary proteins rebinding experiment.•CA/PAM MIP is low-cost, easy to be prepared and has potential application in cell culture.•The eluent SDS should be avoided in preparing MIP for cell culture.Protein imprinted calcium alginate/polyacrylamide hydrogel film (CA/PAM MIP) with high toughness was prepared using bovine serum albumin (BSA) as template molecule, sodium alginate and acrylamide as functional monomers, N,N′-methylenebisacrylamide (MBAA) as the covalent cross-linker and CaCl2 as the ionic cross-linker via UV radiation-reduced polymerization. Factors affecting the adsorption capacity and imprinting efficiency of the BSA-imprinted CA/PAM hydrogel films were investigated, such as ratio of polyacrylamide/sodium alginate, film thickness, MBAA concentration and CaCl2 concentration. Results showed that the CA/PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA compared with non-imprinted polymer (NIP). The adsorption capacity of MIP for BSA reached 22.49 mg/g, which was 2.7 times higher than NIP. The regeneration property of the BSA-imprinted CA/PAM hydrogel was distinctly improved and the imprinting efficiency of CA/PAM MIP maintained 77.95% of the initial value after five repetitions. Single and binary proteins rebinding indicated that the CA/PAM MIP exhibited good recognition performance. Cell culture experiments showed CA/PAM MIP was more suitable for cell culture than CA/PAM NIP. The residual sodium dodecyl sulfate (SDS) in the elution process leaded to the death of mouse fibroblast cells (L929) after 3 days. A moderate elution solution without residue eluent should be used to prepare MIP for cell culture.